We offer three categories:
- Stage I* – Grid/Ice Optimization: process of finding conditions that produce grids with ideal ice thickness given the combination of sample, buffer, and freezing apparatus.
- Stage II* – Sample Optimization: screening grid substrates, detergents, or other additives to optimize sample distribution on a grid. PNCC does not offer sample preparation or purification optimization services but does offer consultations with staff.
- Stage III – Grid Prioritization: identifies which grids would be ideal for high-resolution data collection (i.e. “Krios-ready” grids, see “Microscope Services”). at this stage of screening are expected to have already moved through ice and sample optimization.
All stages are prioritized based on access to cryoEM resources.